Y-27632의 치과용 레진 단량체 유래 세포독성에 대한 치주줄기세포 보호 효과

• 영문명: The Protective Effects of Y-27632 on Periodontal Ligament Stem Cells against Cytotoxicity Induced by Dental Resin Monomers
• 발행기관: 한국구강보건과학회
• 저자명: 박소영(So Yeong Park)
• 간행물 정보: 『한국구강보건과학회지』제13권 제2호, 38~45쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

Objectives: Triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA), widely used monomers in dental resin materials, have been reported to induce cytotoxic effects in various cell types. This study aimed to investigate the cytotoxic effects of TEGDMA and HEMA on human periodontal ligament stem cells (PDLSCs) and to evaluate the protective role of the Rho-associated kinase inhibitor Y-27632. Methods: PDLSCs were isolated from premolars and cultured under standard conditions. Cells were exposed to various concentrations (1, 2.5, 5, 10 mM) of TEGDMA and HEMA for 6 or 24 hours. Y-27632 was applied at concentrations ranging from 1 to 25 μM to assess its effect on cell viability and cytotoxicity. To evaluate the protective effect, PDLSCs were treated with 5 μM Y-27632 following exposure to 5 mM TEGDMA or HEMA. Cell viability was analyzed using the WST-1 assay. Results: Both TEGDMA and HEMA reduced PDLSC viability in a dose- and time-dependent manner. TEGDMA showed greater cytotoxicity than HEMA. Y-27632, at lower concentrations (1-5 μM), did not exhibit toxicity and significantly improved cell survival when applied after monomer exposure. The protective effect is likely mediated by the suppression of reactive oxygen species generation and the inhibition of caspase-related apoptotic pathways. Conclusions: Y-27632 effectively attenuates TEGDMA- and HEMA-induced cytotoxicity in PDLSCs by modulating apoptotic signaling. These findings suggest the potential of Y-27632 as an adjunctive agent to improve the biocompatibility of dental resin materials. Further in vivo studies are necessary to confirm its clinical applicability

목차

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 연구결과

Ⅳ. 고찰

Ⅴ. 결론

REFERENCES

키워드

2-hydroxyethyl methacrylate periodontal ligament stem cells resin monomers triethylene glycol dimethacrylate Y-27632