구조생물학을 위한 Cryo-EM Grid 준비 전략: 재료부터 시료 준비까지

• 영문명: Preparation Strategies for Cryo-EM Grids for Structural Biology: from Materials to Preparation
• 발행기관: 한국세라믹학회
• 저자명: 김지수(Jeesoo Kim)
• 간행물 정보: 『세라미스트』제28권 제2호, 281~293쪽, 전체 13쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

Cryo-electron microscopy (Cryo-EM) has revolutionized structural biology, enabling near-atomic resolution analysis of complex biomolecules, particularly membrane proteins and dynamic assemblies. While advances in direct electron detection and motion correction algorithms have significantly improved image quality, specimen preparation remains a major bottleneck in achieving reproducible high-resolution structures. This review highlights recent innovations in cryo-EM grid materials, including gold grids, graphene-based supports, and self-wicking nanowire grids, which improve sample stability, ice thickness uniformity, and beam-induced motion suppression. Surface functionalization strategies such as PEGylation and affinity coatings further enhance particle distribution and reduce adsorption artifacts. Moreover, novel vitrification platforms with real-time suction control and intelligent design offer improved reproducibility and throughput. These integrated developments in grid engineering and sample handling are poised to transform cryo-EM workflows, enabling more consistent, efficient, and high-resolution structural studies for a wide range of biological targets.

목차

1. 서론

2. 본론

3. 결론

REFERENCES

키워드

Cryo-EM Gold Graphene Grid engineering Structural biology