섬유주세포에서 Dipyridamole이 활성산소종과 산화스트레스에 미치는 영향

• 영문명: Effect of Dipyridamole on the Reactive Oxygen Species and Oxidative Stress in Trabecular Meshwork Cells
• 발행기관: 대한안과학회
• 저자명: 이근우 김재우,Keun Woo Lee, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume54,number3, 496~501쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.03.15

영문 초록

Purpose: To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC). Methods: Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 μm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay. Results: DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides. Conclusions: DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.

키워드

Antioxidant, Dipyridamole, Reactive oxygen species, Trabecular meshwork cells