각막내피세포의 증식 및 손상의 회복에 대한 ROCK 억제제 Y-27632의 효과

• 영문명: Effect of ROCK Inhibitor on the Expansion and Wound Healing of Human Corneal Endothelial Cell
• 발행기관: 대한안과학회
• 저자명: 김경환 정진권 류진숙 고아영 김미금 위원량,Kyeong Hwan Kim, MD,, Jin Kwon Chung, MD,, Jin Suk Ryu, MS, Ah Young Koh, BS,Mee Kum Kim, MD, PhD,, Won Ryang Wee, MD, PhD,
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume54,number3, 479~489쪽, 전체 11쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2013.03.15

영문 초록

Purpose: To investigate the effect of ROCK inhibitor Y27632 on the human corneal endothelial cell proliferation in vitro and in vivo. Methods: Using corneal endothelial cells isolated and cultured from human donor cornea, we compared the effect of Y27632 (10 μM ) on the proliferation in vitro by flow cytometry analysis. For the evaluation of the effect of Y27632 (10 mM) in vivo, corneal thickness and wound area were analyzed for the corneal endothelial wound rabbit model induced by transcorneal freezing. Results: Ki67 positive cells were increased in the Y27632 group (9.1 ± 4.1 %) than the control group (8.0 ± 5.9 %), whereas annexin V positive cells in the Y27632 group (2.9 ± 1.0 %) were decreased compared to the control group (4.2 ± 2.2 %). However these were not statistically significant. Wound area after Y27632 application in animal model is concerned, the control group showed significant smaller area (45.6 ± 0.6 mm2) compared to the Y27632 group (49.3 ± 0.8 mm2; p = 0.029, Mann-Whitney U test), however, these were not significantly different from the baseline. Corneal thickness was not different between the two groups. Conclusions: Different from other reports for the effect of Y27632, no significant effect on the proliferation in vitro and wound healing in vivo, regarding human corneal endothelial cell, were found in this study.

키워드

Human corneal endothelium, Rho-associated kinase inhibitor, ROCK inhibitor, Wound healing, Y27632