초유에 함유되어 있는 면역조절물질인 MIEF가 B 세포의 분화에 미치는 영향

• 영문명: Induction of B Lymphocyte Differentiation by a Colostral Immunomodulatory Protein MIEF
• 발행기관: 대한약학회
• 저자명: 이종호(Jong Ho Lee) 이종길(Chong Kil Lee) 한성순(Seong Sun Han)
• 간행물 정보: 『약학회지』제38권 제3호 (1994년), 351~357쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.06.30

영문 초록

The levels of maternal immunity enhancing factor(MIEF), which is an immunomodulatory protein identified from bovine colostrum, were determined by indirect competitive enzyme-linked immunosorbent assay(ELISA) for the colostrum and normal milk collected during the first two weeks of lactation. The mean concentration of MIEF in the colostrum of the first day of lactation was 109mcg/ml, and fell from the third day of lactation to 3-4mcg/ml. The molecular weight of the purified MIEF determined by reducing SDS-PAGE and TSK G2000SW column chromatography was 22,000 and 24,000 daltons, respectively, showing that MIEF is a monomeric peptide in its native form. To examine the capacity of MIEF to induce differentiation of B lymphocytes, human tonsillar lymphocytes were cultured in the presence of different concentrations of MIEF, and then antibody secreting cells were enumerated by enzyme-linked immunospot(ELISPOT) assay. When added to cultures of human tonsillar lymphocytes, MIEF induced differentiation of resting B lymphocyte to antibody secreting plasma cells as efficiently as LPS.

키워드

Colostrum Maternal immunity enhancing factor(MIEF) Indirect competitive enzyme-linked immunosorbent assay(ELISA) Enzyme-linked immunospot(ELISPOT) assay B lymphocyte differentiation.