마크로라이드-린코사마이드-스트렙토그라민 B(MLS)계 항생물질에 대한 유도 내성

• 영문명: Screening of Inducible Resistance Genes to Macrolide-Lincosamide-Streptogramin B(MLS) Antibiotics
• 발행기관: 대한약학회
• 저자명: 권애란(Ae Ran Kwon) 최성숙(Sung Sook Choi) 김숙경(Sook Kyung Kim) 정영자(Young Ja Chung) 최응칠(Eung Chil Choi) 김병각(Byoung Kak Kim)
• 간행물 정보: 『약학회지』제38권 제3호 (1994년), 293~299쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1994.06.30

영문 초록

Forty nine clinical isolates of S. aureus showing resistance to erythromycin(EM) were selected from 83 strains isolated recently in Korea. Fourteen strains of S. aureus showing inducible resistance to MLS antibiotics were selected by disc agar diffusion method. Colony hydridization was executed using two MLS inducible resistance genes, ermA and ermC, identified previously from S. aureus as probes. S. aureus 375 and S. aureus 507 whose genes were not homologous to those probes were finally selected. It was confirmed that the resistance genes of S. aureus 375 and S. aureus 507 had no homology with those probes in southern hybridization test using ermA, ermC and ermAM as probes. It was determined that S. aureus 375 had a plasmid whose size was about 35 kb. To know if the plasmid may have the genes related to inducible resistance to MLS antibiotics, it was attempted to transform Bacillus subtillis BR151 and S. aureus RN4220 with the plasmid isolated from S. aureus 375. It was shown that the gene related to inducible resistance to MLS antibiotics did not exist in this plasmid. These results indicate that two clinical isolates of S. aureus showing inducible resistance to MLS antibiotics have novel genes that have no homology with MLS resistance genes identified so far. It is assumed that these genes may exist in chromosomal DNA.

키워드

S. aureus MLS antibiotics inducible resistance colony hybridization southern hybridization B. subtilis BR151 S. aureus RN4220 transformation.