랫드 척수후각 단일세포 분리 및 특성에 관한 연구

• 영문명: Isolation and Electrical Characterization of the Rat Spinal Dorsal Horn Neurons
• 발행기관: 대한약리학회
• 저자명: 한성규(Seong Kyu Han) 류판동(Pan Dong Ryu)
• 간행물 정보: 『대한약리학잡지』제32권 제2호, 283~292쪽, 전체 10쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.09.30

영문 초록

The spinal dorsal horn is the area where primary afferent fibers terminate and cutaneous sensory information is processed. A number of putative neurotransmitter substances, including excitatory and inhibitory amino acids and peptides, are present in this region. In this study, single neurons of the spinal dorsal horn were acutely isolated and the properties of whole cell current and responses to excitatory and inhibitory neurotransmitters were studied by patch clamp technique. Transverse slice ((300μm) of lumbar spinal cords from young rats(7 ~ 14 days) were sequentially treated with two pretenses(pronase 0.75 mg/ml and thermolysin 0.75 mg/ml), then single neurons were mechanically dissociated. These neurons showed near-intact morphology such as multipolar, ellipsoidal and bipolar, and pyramidal cells and we recorded the typical whole cell currents of K⁺, Ca²⁺ and ligand-operated channels from these neurons. Glutamate (30μM) and N-methyl-D-aspartate(NMDA, 30μM) induced inward currents of 117 ± 12.4 pA(n=5) and 49 ± 6.9 pA(n=3), respectively. Glycine (1μM) potentiated glutamate-induced currents 4 ~ 5 times and NMDA-induced currents 8 ~ 10 times. In addition, glycine (30μM) induced Inward current (31 ± 6.1 nA, n=2), which was rapidly desensitized after the peak to a new steady-state level. However, the inward currents induced by γ-amino butyric acid(GABA, 1μM) decreased continuously after the peak(226 ± 41.6 pA, n=3) under the similar experimental condition. The ionic currents and pharmacological responses of isolated neurons in this work were similar to those observed in vivo or in vitro spinal cord slice, indicating that acutely isolated neurons could be effectively used for further pharmacological studies.

키워드

Spinal dorsal horn Excitatory and inhibitory amino acids acutely-isolated neurons