흰쥐 대뇌피질 신경세포에 미치는 호모시스틴의 신경독성에 대한 S-nitrosation의 역할

• 영문명: S-nitrosation Ameliorates Homocysteine-mediated Neurotoxicity in Primary Culture of Bat Cortical Neurons
• 발행기관: 대한약리학회
• 저자명: 김원기(Won-Ki Kim)
• 간행물 정보: 『대한약리학잡지』제32권 제2호, 169~176쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1996.09.30

영문 초록

The reactivity of the sulfhydryl (thiol) group of homocysteine has been associated with an Increased risk of atherosclerosis, thrombosis and stroke. Thiols also react with nitric oxide (NO, an endothelium-derived relaxing factor (EDRF) ), forming S-nitrosothiols that have been reported to have potent vasodilatory and antiplatelet effects and been expected to decrease adverse vascular effects of homocysteine. The present study was aimed to Investigate whether the S-nitrosation of homocysteine modulates the neurotoxic effects of homocysteine. An 18 hour-exposure of cultured rat cortical neurons to homocysteine ( >1 mM) resulted in a significant neuronal cell death. At comparable concentrations ( <10 mM), however, S-nitrosohomocysteine did not induce neuronal cell death. Furthermore, S-nitrosohomocysteirle partially blocked NMDA-mediated neurotoxicity. S-nitrosohomocysteine also decreased NMDA-mediated increases in intracellular calcium concentration. The present data indicate that in brain nitric oxide produced from neuronal and nonneuronal cells can modulate the potential, adverse properties of homocysteine.

키워드

Homocysteine S-nitrosohomocysteine Neurotoxicity Cortical neuron Primary culture Calcium