Steroidal Saponin을 이용한 위유, 황정의 분류 및 함량 분석법 개발

• 영문명: Content Analysis and Classification for Polygonati Odorati Rhizoma and Polygonati Rhizoma by Steroidal Saponin
• 발행기관: 대한약학회
• 저자명: 김성건(Sungun Kim) 신소영(So Young Shin) 문예지(Ye Ji Moon) 서지윤(Ji Yoon Seo) 김호경(Ho Kyoung Kim) 황완균(Wan Kyunn Whang)
• 간행물 정보: 『약학회지』제54권 제6호 (2010년), 441~448쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.12.31

영문 초록

In present study, classification and quality control of Genus Polygonatum were developed using the isolated from Polygonati Odorati Rhizoma and Polygonati Rhizoma. 3 components were isolated from Butanol fractions of Polygonati Rhizoma, and 2 components were isolated from Hexane and Butanol fractions of Polygonati Odorati Rhizoma. All the components were obtained using silica gel and ODS column chromatography. The compounds were identified as adenosine, 14-hydroxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranosyl-26-O-β-D-glucopyranoside, 22-O-methyl-14-hydrocxyfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-Dgalactopyranosyl-26-O-β-D-glucopyranoside, β-Sitosteryl-3-O-β-D-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-β-Dglucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranoside through physicochemical data, spectroscopic methods (1H-NMR, 13C-NMR, Mass) according references. The quality control of genus Polygonatum were conducted using HPLC quantitative analysis of 14-hydroxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranosyl-26-O-β-D-glucopyranoside, 14-hydoxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranoside in 30 samples collected throughout Korea and China. This method provided a tool for standardization of mix or misusing the commercial Odorati Rhizoma and Polygonati Rhizoma. As a result, contained quantity of 14-hydroxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl-(1→4)-O-β-D-galactopyranosyl-26-O-β-D-glucopyranoside was measured 0.008±0.006% and 14-hydoxylfurost-5-ene-3-O-β-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(13)]-O-β-D-glucopyranosyl-(1→4)-O-β-Dgalactopyranoside was measured 0.026±0.012%.

키워드

Polygonati rhizoma Polygonati odorati rhizoma steroidal saponin origin content analysis