HS-SPME-GC/MS에 의한 혈액중 17종 유기인계 농약의 동시분석법

• 영문명: Simultaneous Analysis of 17 Organophosphorous Pesticides in Blood by Automated Head Space-SPME GC/MS
• 발행기관: 대한약학회
• 저자명: 이종숙(JongSook Rhee) 정진미(JinMi Jung) 이한선(HanSun Lee) 염혜선(HyeSun Yeom) 이상기(SangKi Lee) 박유신(YooSin Park) 정희선(HeeSun Chung)
• 간행물 정보: 『약학회지』제54권 제6호 (2010년), 429~440쪽, 전체 12쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2010.12.31

영문 초록

HS-SPME-GC/MS was studied and optimized for the determination of 17 orgarnophosphorous pesiticides (OPPs: chlorpyrifos, chlorpyrifos-methyl, demeton-s-methyl, diazinon, dimethoate, EPN, fenitrothion, fenthion, malathion, methidathion, monocrotophos, parathion, phenthoate, phosphamidon, sulfotep, terbufos, triazophos) in blood. Optimum SPME parameters were selected: choice of SPME fiber (85 μm polyacrylate), pH effect (0.5 N HCl), salt effect (Na2SO4, 0.2 g; 20%), headspace incubation temperature (80oC), headspace incubation time (1 min), headspace adsorption time (30 min) and GC desorption time (2 min). These parameters were optimized using HS-SPME autosampler coupled with gas chromatography-mass spectrometry (GC-MS). Method validation was carried out in terms of linearity, limit of detection (LOD), limit of quantitation (LOQ) and recovery in blood. The assay was linear over 0.5~5.0 mg/l (r2=0.955~1.000). Limit of detection (LOD) and limit of quantitation (LOQ) in blood were determined 0.03~0.3 mg/l (S/N=3) and 0.1~1.1 mg/l (S/N=10), respectively. Relative recovery with 0.5, 1 and 5 mg/l (in blood) were 90.8%, 98.5% and 94.1%, respectively. This method will be applied to the determination of the orgarnophosphorous pesticides in postmortem blood. The proposed protocol can be an attractive alternative to be used in routine toxicological analysis.

키워드

pesiticide HS-SPME-GC/MS polyacrylate fiber blood