능소화의 꽃받침으로부터 Protein Kinase C저해물질인 Verbascoside의 분리 및 그 생물활성

• 영문명: Isolation and Biological Activity of Verbascoside, A Potent Inhibitor of Protein Kinase C from the Calyx of Campsis grandiflora
• 발행기관: 대한약학회
• 저자명: 이현선(Hyun Sun Lee) 박문수(Moon Su Park) 오원근(Won Ken Oh) 안순철(Soon Cheol Ahn) 김보연(Bo Yeon Kim) 김환묵(Hwan Mook Kim) 오구택(Goo Taeg Oh) 민태익(Tae Ick Mheen) 안종석(Jong Seog Ahn)
• 간행물 정보: 『약학회지』제37권 제6호 (1993년), 598~604쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.12.30

영문 초록

The calyx extract of Campsis grandiflora displayed inhibitory activity against protein kinase C from the bovine brain. Separation guided by protein kinase C enzyme assay and bleb forming assay led to isolation of a potent protein kinase C inhibitor that was identified as a known phenylpropanoid glycoside, verbascoside. It suppressed completely bleb-formation of K562 cell surface induced by phorbol 12,13-dibutylate at the concentration of 60mcg/ml and IC50 of the protein kinase C occured at 20mcM. This compound was tested for cytotoxic activity against ten human tumor cell lines in vitro. it exhibited moderate cytotoxic activity against skin tumor cell line M14 (IC50 2.2mcg/ml) and very weak cytotoxicity against other cell lines(IC50>10mcg/ml)

키워드

Campsis grandiflora verbascoside protein kinase C inhibitor anti-bleb forming activity cytotoxicity human tumor cell lines.