Antioxidant Effects of Pyrogallol in Neural Cells and Mice Brain

• 발행기관: 한국교원대학교 뇌·AI기반교육연구소
• 저자명: 문지섭(Jisub Moon) 곽희선(Heesun Gwak) 이태희(Tae-Hee Lee) 안은석(Eun Suk An) 김윤배(Yun-Bae Kim) 박동선(Dongsun Park)
• 간행물 정보: 『Brain, Digital, & Learning』제9권 제3호, 113~124쪽, 전체 12쪽
• 주제분류: 사회과학 > 교육학
• 파일형태: PDF
• 발행일자: 2019.09.30

영문 초록

Polyphenols can easily react with reactive oxygen species (ROS) resulting in powerful antioxidant activity. Pyrogallol is a polyphenol found in a variety of fruits and vegetables. Therefore, plants containing pyrogallol can be used for their antioxidant, anti-bacterial, anti-fungal, anti-allergic, and anti-inflammatory properties. However, its effectiveness as an antioxidant is still a matter of debate because pyrogallol is also a superoxide anion generator and induces superoxide anion-mediated death of several types of cells. In this study, we tried to confirm the antioxidant effects and mechanisms of pyrogallol in mouse neuroblastoma (N2A), human neural stem cells (F3), and mouse brain. Treatments with various concentrations of pyrogallol significantly increased the free radical scavenging ability and reduced lipid peroxidation in N2A and F3 cells in a concentration-dependent manner. In the cytotoxicity test, low concentrations of pyrogallol (0–10 µg/mL) did not significantly influence cell viability, while high concentrations (125-1000 µg/mL) induced death in N2a and F3 cells. Furthermore, treatment with pyrogallol(1.0, 3.2, and 10.0 µg/mL) up-regulated the mRNA expression of superoxide dismutases 1, 2, and 3, glutathione peroxidase (GPx)1, and phospholipid hydroperoxide GPx in N2A and F3 cells, in the brains of mice that were orally administered pyrogallol (1.0, 3.2, and 10.0 mg/kg/day) for 7 days. The mice did not exhibit any toxic effects due to this treatment. Taken together, although pyrogallol induced cell death at high concentrations, it might be a good candidate antioxidant for neurodegenerative disease at low concentrations.

목차

I. Introduction

II. Materials and Methods

III. Results

IV. Conclusions

키워드

Pyrogallol antioxidant reactive oxygen species free radical neuroprotection