유전자총에 의한 담배의 형질전환

• 영문명: Transformation of Tobacco by Gene-gun
• 발행기관: 한국자원식물학회
• 저자명: 김병오(Byung-Oh Kim) 김경민(Kyung-Min Kim) 오중열(Jung-Youl Oh)
• 간행물 정보: 『한국자원식물학회지』제21권 제4호, 249~253쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.08.31

영문 초록

Since there is no report about more than one gene expression simultaneously in a single mitochondria, this report is very important to novel type of eukaryotic gene expression. In this study, investigated whether mitochondrial expressed gene and GFP that expression in mitochondria of plant expressed to mitochondria. Expression vector (pBin) containing AtBI-1 (mitochondrial expressed gene) and GFP driven by 35S promoter was bombarded by gene gun to leaves and cotyledon of tobacco. Regenerated shoot confirmed expression of AtBI-1 in mitochondria by GFP expression, PCR, and Southern analysis. Successful mitochondria of plant cell transformation in this report implies possible eukaryotic mitochondrial transformation including plants and animals, and moreover two or more gene expression which can be excellent applicable protocols to pharmaceutical field including antibody production.

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키워드

Tobacco Gene-gun Mitochondria