Selection of null genotype without four proteins (KTI, lectin, P34, and 7S α′ subunit) in black soybeans with green cotyledons

• 발행기관: (사)한국식물생명공학회
• 저자명: Byeong Eon Park Sarath Ly Jae Gyeong Cho Jin Young Moon Jong Il Chung
• 간행물 정보: 『Journal of Plant Biotechnology』52권 2호, 107~112쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

Black-soybean seeds with green cotyledons are widely used owing to their high levels of bioactive compounds such as anthocyanin and lutein. However, they also contain antinutritional components (e.g., the KTI, lectin, P34, and 7S-α′-subunit proteins) that reduce the nutritional and functional value of the seeds. This study aimed to select a new, improved soybean line with black seed coats and green cotyledons and without the KTI, lectin, P34, and 7S-α′ -subunit proteins (i.e., with the titilelep34p34cgy1cgy1 genotype). Using four germplasms, a breeding population and 151 F2 seeds with black seed coats and green cotyledons were obtained. Nine F2 seeds with the recessive titip34p34cgy1cgy1 genotype were selected and planted in a field to grow F2 plants. Two F2 plants with the lele genotype were identified, after which one plant with elite agronomic traits was selected. The titilelep34p34cgy1cgy1 genotype was confirmed in random F3 seeds harvested from the selected F2 plant. The seeds sown in the field displayed a short stem length and a neutral 100-seed weight of 48 cm and 26.5 g, respectively. Meanwhile, the color of their seed coats was black, and that of the cotyledons was green. This newly selected line can be used as a parent line to breed cultivars of varied colors, with green cotyledons, and without the KTI, lectin, P34, and 7S- α′-subunit antinutrient proteins.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

References

키워드

KTI lectin P34 7S α′ subunit black seed coat green cotyledon soybean