당뇨망막병증에서 말초혈액의 내피전구세포의 관여

• 영문명: Involvement of Circulating Endothelial Progenital Cells and Vasculogenic Factors in Diabetic Retinopathy
• 발행기관: 대한안과학회
• 저자명: 이인걸 김재찬,In Gul Lee, M.D., Jae Chan Kim, M.D., Ph.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume46,number2, 271~278쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2005.02.28

영문 초록

Purpose: Retinal neovascularization in diabetes has been thought to follow the release of local angiogenic factors in the retina. We hypothesized that neovascularization of diabetic retinopathy is a systemic vasculogenesis rather than a local angiogenesis. Thus, we evaluated the concentrations of circulating endothelial progenitor cells (EPCs) and stem cell modulation factors such as vascular endothelial growth factor (VEGF), erythropoietin (EPO), and substance p (SP) in the peripheral blood of diabetic retinopathy patients. Methods: We studied 15 normal controls and 45 Type 2 diabetic patients [non-DR group (n=15), NPDR group (n=15), and PDR group (n=15)]. We measured circulating CD34+mononuclear cells (CD34+MNCs) and c-Kit+ mononuclear cells (c-Kit+MNCs) by flow cytometry. VEGF, EPO and SP in the peripheral blood were measured by ELISA. Results: The circulating CD34+MNCs and c-Kit+MNCs increased in the NPDR and PDR groups compared with the control group (P<0.01). The serum level of VEGF was increased in the NPDR and PDR groups compared with the control group (P<0.05). The level of EPO was exclusively elevated in the non-DR group compared with the other three groups (P<0.01). The circulating SP level increased in the NPDR and PDR groups compared with the control group (P<0.05). Conclusions: The present study is the first to demonstrate that CD34+MNCs, c-Kit+MNCs and their modulator are elevated in diabetic retinopathy patients. Therefore, it is possible that circulating EPCs and serum VEGF, EPO and SP may be involved in the progression of diabetic retinopathy.

키워드

Diabetic retinopathy, Endothelial progenitor cells, Erythropoietin, Substance P, Vascular endothelial growth factor