Further Characterization of Bombesin Like Immunoreactivities from the Skin of Korean Fire-bellied Toad, Bombina orientalis

• 영문명: Further Characterization of Bombesin Like Immunoreactivities from the Skin of Korean Fire-bellied Toad, Bombina orientalis
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Hyeok Yil Kwon Hyung Seo Park Moo Ho Won Yun Lyul Lee Hyoung Jin Park
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제2권 제5호, 637~644쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1998.01.01

영문 초록

Previously, we have isolated authentic bombesin and another bombesin like peptide named bombesin like immunoreactivity (BLI)-K2 from the skin of Korean fire-bellied toad, Bombina orientalis. In the present study, we have newly purified three heterogeneous forms of BLI named BLI-K3, BLI-K4, and BLI-K5 from side fractions obtained in previous isolation of bombesin like peptide. The BLIs were separated into five peaks on a column of C₁₈ preparative HPLC. Among them, three minor peaks containing BLI-K3, K4, and K5 were purified by means of sequential chromatography on the columns of SP cation exchange HPLC and C₁₈ reverse phase HPLC. The purified BLI-K3 and K4 showed high binding affinity to an anti-bombesin serum (LBE 2G-2) with binding potency of 72 and 95%, respectively, relative to that of bombesin. However, they did not possess any distinctive biological activity of bombesin like peptide. On the contrary, the biological activity of BLI-K5 was similar to that of bombesin but its binding affinity to an anti-bombesin serum was low. The results indicate that three heterogeneous forms of BLI were coexpressed with bombesin and BLI-K2 in the skin of B. orientalis. All forms of the purified BLI in the present study were immunologically active but only BLI-K5 possessed the distinctive biological activity of bombesin like peptide.

키워드

Bombesin Bombesin like immunoreactivity Frog skin peptide Molecular heterogeneity Gastrin release Pancreatic exocrine secretion