Altered Cerebral Vasomotion with Decreased CGRP Level in Pial Arteries of Spontaneously Hypertensive Rats

• 영문명: Altered Cerebral Vasomotion with Decreased CGRP Level in Pial Arteries of Spontaneously Hypertensive Rats
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Kwang Ho Lee Jae Moon Choi Ki Whan Hong
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제2권 제5호, 573~580쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1998.01.01

영문 초록

The study aims to identify the mechanism (s) underlying the altered vasodilatory responses of the pial artery of spontaneously hypertensive rats (SHR) under a hypothesis that calcitonin gene-related peptide (CGRP) exerts a modulator role in the autoregulation of cerebral blood flow (CBF). The animals were divided into four groups: 1) Sprague-Dawley rats (SDR), 2) Wistar rats (WR), 3) SHR with high blood pressure (BP≥150 mmHg), and 4) SHR with normotensive BP (≤150 mmHg). The lower limit of CBF autoregulation in SHR shifted to a higher BP (82.8⁑9.3 mmHg, P＜0.05) than that in SDR (58.9⁑5.7 mmHg). In SHR, whether the BP levels were high or normotensive, the vasodilator responses to a stepwise hypotension were significantly attenuated unlike with SDR and WR. When artificial cerebrospinal fluid (CSF) containing capsaicin (3⁓10^-7 M) was suffused over the cortical surface, a transient increase in pial arterial diameter was observed in the SHR with high or normotensive BP. In contrast, SDR and WR showed a large increase in diameter, and the increase was sustained for over 10 minutes. In line with these results, the basal releases of CGRP-like immunoreactivity (CGRP-LI) in the isolated pial arteries from SHR with high and normotensive BP were 12.5⁑1.4 and 9.8⁑2.8 fmole/mm²/60 min (P＜0.05), while those from SDR and WR were 25.5⁑3.1 and 24.6⁑3.1 fmole/mm²/60 min, respectively. The isolated basilar arteries showed similar results to those of the pial arteries in SHR. Thus, it is summarized that, in the SHR, the reduced autoregulatory vasodilator responses to stepwise hypotension and capsaicin may be, in part, ascribed to the decreased release of CGRP from the perivascular sensory nerve fibers of the pial arteries, and that altered vasomotor activity in SHR may not be related with the hypertensive tone.

키워드

Cerebral autoregulation CGRP Spontaneously hypertensive rats Cerebral blood flow Capsaicin