Role of Phospholipase A2 in Oxidant-induced Alteration in Phosphate Transport in Primary Cultured Rabbit Renal Proximal Tubule Cells

• 영문명: Role of Phospholipase A₂ in Oxidant-induced Alteration in Phosphate Transport in Primary Cultured Rabbit Renal Proximal Tubule Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Kwon Moo Park Sun Hee Ko Jae Suk Woo Jin Sup Jung Sang Ho Lee Yong Keun Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제2권 제5호, 601~609쪽, 전체 9쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1998.01.01

영문 초록

The present study was undertaken to examine the role of phospholipase A₂ (PLA₂) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release in a dose- dependent fashion. A PLA₂ inhibitor mepacrine prevented tBHP-induced AA release, but it did not alter the inhibition of phosphate uptake and the decrease in cell viability induced by tBHP. tBHP-induced inhibition of phosphate transport was not affected by a PKC inhibitor, staurosporine. tBHP at 0.1 mM did not produce the inhibition of Na^⁢-K^⁢-ATPase activity in microsomal fraction, although it significantly inhibited at 1.0 mM. These results suggest that tBHP can inhibit phosphate uptake through a mechanism independent of PLA₂ activation, irreversible cell injury, and lipid peroxidation in primary cultured rabbit renal proximal tubular cells.

키워드

Phosphate uptake Oxidant PLA₂ Proximal tubular cells