[3H]MK-801 Binding to the Synaptic Membranes of Rat Forebrains Age-related Regulation by Glutamate, Glycine and Spermine

• 영문명: [³H]MK-801 Binding to the Synaptic Membranes of Rat Forebrains Age-related Regulation by Glutamate, Glycine and Spermine
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Jungsook Cho Jae Yang Kong
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제2호, 117~125쪽, 전체 9쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

The N-methyl-D-aspartate (NMDA) receptor-mediated glutamatergic neurotransmission is involved in synaptic plasticity, developmental processes, learning and memory and many neuropathological disorders including age-related diseases. In the present study, regulation of the NMDA receptor properties by various ligands was investigated using [³H]MK-801 binding studies in the synaptic membranes of young and aged rat forebrains. The binding in the presence of glutamate and glycine increased dramatically with growth between 1 and 6 weeks old, and thereafter declined gradually with aging. Glutamate, glycine or spermine respectively increased the binding with growth. Glutamate maintained the binding during aging, while glycine or spermine significantly decreased the binding in the aged brain. The maximum stimulation by glycine varied depending on the ages of brains. Greater sensitivity to glycine was observed at 1 week and 3 months and the sensitivity was significantly reduced in the aged brain. In contrast, spermine showed similar stimulation patterns in young and aged rats. These results indicated that the functional properties of the NMDA receptor-ion channel complex in young and aged rat forebrains are differentially regulated by agonists, and the reduction of the receptor function with normal aging may be, in some degree, due to the reduction of the receptor sensitivity to glycine.

키워드

NMDA receptor Aging Synaptic plasticity Radioligand binding MK-801 Abbreviations NMDA N-methyl-D-aspartate EAA excitatory amino acid LTP long-term potentiation PCP phencyclidine MK-801 (+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cycl