Effect of Hemorrhage on mRNA Expressions of Renin, Angiotensinogen and AT1 Receptors in Rat Central and Peripheral Tissues

• 영문명: Effect of Hemorrhage on mRNA Expressions of Renin, Angiotensinogen and AT₁ Receptors in Rat Central and Peripheral Tissues
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Mi Kyung Lee Hakryul Jo Kyung-Soon Kim Eun-Kyoung Yang Won-Jung Lee
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제1권 제2호, 151~159쪽, 전체 9쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1997.01.01

영문 초록

In an attempt to investigate whether hemorrhage affects the gene expression of the renin-angioteusin system (RAS) components in the brain and peripheral angiotensin-generating tissues, changes in mRNA levels of the RAS components in response to hemorrhage were measured in conscious unrestrained rats. Wistar rats were bled at a rate of 3 ml/kg/min for 5 min, and then decapitated 7 h after hemorrhage. Levels of mRNA for renin, angiotensinogen and angiotensin II-AT₁ receptor subtypes (AT_1A and AT_1B) were determined with the methods of northern blot and reverse transcriptase-polymerase chain reaction (RT-PCR). Hemorrhage produced a profound hypotension with tachycardia, but blood pressure and heart rate recovered close to the basal level at 7 h. Plasma and renal renin levels were significantly increased at 7 h. Hemorrhage induced rapid upregulation of gene expression of both AT_1A and AT_1B receptor subtypes in the brainstem and hypothalamus, downregulation of them in the adrenal gland and liver. However, renin mRNA level increased in the brainstem, decreased in the liver, but was not changed in the hypothalamus, kidney and adrenals after hemorrhage. Angiotensinogen mRNA level was not significantly changed in any of the tissue except a slight increase in the liver. The kidney and liver did not show any significant change in gene expression of the RAS components. These results suggest that gene expression of the RAS in central and peripheral tissues are, at least in part, under independent control and the local RAS in each organ plays specific physiologic role.

키워드

Northern blot RT-PCR Brain Kidney Liver Adrenal