서울시 수계시설에서 분리된 Legionella pneumophila의 분자역학적 특성

• 영문명: Molecular Epidemiology of Legionella pneumophila Isolated from Water Supply Systems in Seoul, Korea
• 발행기관: 한국환경보건학회
• 저자명: 전수진(Su Jin Jeon) 정지헌(Ji Hun Jung) 승현정(Hyun Jung Seung) 김창규(Chang Kyu Kim) 진영희(Young Hee Jin) 오영희(Young Hee Oh) 최성민(Sung Min Choi) 채영주(Young Zoo Chae)
• 간행물 정보: 『한국환경보건학회지』제39권 제2호, 166~177쪽, 전체 12쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2013.04.30

영문 초록

Objectives: The genus Legionella is common in aquatic environments. Some species of Legionella are recognized as potential opportunistic pathogens for human, notably Legionella pneumophila that causes, Legionellosis. Thus, we investigated the contamination of Legionella pneumophila on water supply systems in Seoul, including cooling towers, public baths, hospitals and fountains. Methods: The existence of 16S rRNA and mip gene of L. pneumophila was confirmed in the genome of the isolated strains by PCR. Results: During the summer season of 2010 and 2011, Legionella pneumophila were detected from 163 samples (21.1%) out of 772 samples collected. Among the 163 strains of L. pneumophila, eighty one isolates belonged to serogroup 1 (57.4%), 23 isolates were serogroup 5 (16.3%), 21 isolates were serogroup 6 (14.9%), 8 isolates were serogroup 2 (5.79%), and 8 isolates were identified in serogroup 3 (5.7%). Through PFGE (pulsed-field gel electrophoresis) analysis using Sfi I, genetic types of L. pneumophila were classified into five (A to E) patterns by the band similarity with excess of 70% from public baths. Conclusions: The PFGE patterns of the serotypes showed a tendency for diversity of L. pneumophila. Our results suggest the existence of serological and genetic diversity among the L. pneumophila isolates.

키워드

Legionella pneumophila mip Pulsed-field gel electrophoresis (PFGE)