HPLC 형광검출법에 의한 Glyphosate의 혈중농도 측정

• 영문명: Determination of Glyphosate in Whole Blood by HPLC -fluorescence Detection
• 발행기관: 대한약학회
• 저자명: 이상기(Sang Ki Lee) 김기욱(Ki Wook Kim) 양자열(Ja Youl Yang) 인상환(Sang Whan In) 이수연(Soo Yeun Lee)
• 간행물 정보: 『약학회지』제45권 제4호 (2001년), 347~351쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2001.08.31

영문 초록

A rapid and sensitive method for the determination of glyphosate, a phosphates amino acid herbicide, in whole blood is presented. After removal of protein, the whole blood was purified by using the anion exchange resin (Dowex 1), and derivatized with 9- fluorenylmethyl chloroformate (FMCL). Derivatized glyphosate from blood sample was injected onto a Whatman partisil 10SAX column and separated with 0.1M phosphate buffer (pH 2.5) and acetonitrile (ratio=3:1). The high performance liquid chromatography- fluorescence detection gave the detection limit of 86pg and linearity of 0.9999 in the range of 0.25μg/ml and 25μg/ml. The recoveries of glyphosate added to the blood samples were ranged from 75.3% to 100.4% compared to the samples prepared in water. The derivatized glyphosate was stable at various acidity and temperature. This method has been successfully applied to the blood samples of lethal intoxication with the herbicide glyphosate.

키워드

Glyphosate HPLC fluorescence detection anion exchange 9-fluorenylmethyl chloroformate.