Hydroperoxide 의존성 반응에서의 Cytochrome P-450의 산화활성종 형성양식

• 영문명: Mechanism of Peroxide-supported Hydroxylation by Cytochrome P-450: Its Formation Pattern of the Active Intermediate
• 발행기관: 대한약학회
• 저자명: 문전옥(Jeon Ok Moon) 김기헌(Ki Heun Kim)
• 간행물 정보: 『약학회지』제37권 제1호 (1993년), 95~99쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1993.02.28

영문 초록

Peroxidase activity of cytochrome P-450 was examined using N, N-dimethylaniline (NDA) as a substrate and cumene hydroperoxide (CHP) as an oxidant. The initial rates of the N-demethylation for varied concentrations of NDA (0.05-0.5 mM) by P-450 at different fixed concentrations of CHP (0.02-0.2 mM) were determined. The results suggest that P-450 proceeds its peroxidative reaction by the rapid equilibrium random bi bi mechanism to form a ternary complex with substrate and oxidant as an active intermediate.

키워드

Cytochrome P-450 Peroxide-supported Hydroxylation rapid equilibrium random bi bi mechanism.