약물과 생체고분자간의 상호작용(II) Difference Spectra에 의한 Cephalothin 및 Cefazoline과 Human Serum Albumin의 결합에 관한 연구

• 영문명: Drug-Biomacromolecule Interactions (II) Binding of Cephalothin and Cefazoline to Human Serum Albumin Using Difference Spectrophotometry
• 발행기관: 대한약학회
• 저자명: 김종국(Chong Kook Kim) 양지선(Ji Sun Yang) 안해영(Hae Young Ahn) 김양배(Yang Bae Kim) 우병설(Byung Sul Yu)
• 간행물 정보: 『약학회지』제25권 제4호 (1981년), 161~165쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 1981.12.30

영문 초록

The binding of two cephalosporins, cephalothin and cefazoline to human serum albumin (HSA) was studied by difference spectrophotometry using a spectrophotometric probe, 2-(4''-hydroxy-benzeneazo) benzoic acid. The probe is strong visible absorbing material which interacts with serum albumin to give characteristic spectrophotometric peaks and provides the basis for a convenient assay to measure free and bound amounts in the presence of serum albumin and competitive drugs. The results obtained showed that the probe and cephalosporin compete for the same binding site on human serum albumin; thus the probe can be used to gauge the displacement of cephalosporins from human serum albumin. The data were interpreted on the basis of theory of multiple equilibria. The number of binding sites of human serum albumin for 2-(4''-hydroxybenzeneazo) benzoic acid(HBAB), cephalothin and cefazoline appears to be 4. By using this technique the binding constants were found as follows: HSA-HBAB, 7.89 X 104M-1; HAS-cephalothin, 1.09 X 103M-1; HAS-cefazoline, 1.21 X 103M-1.