Effects of Plant Oils and Minerals for the Inhibition of Lipase Activity of Staphylococcus aureus Isolated from Fermented Pork Meat

• 발행기관: 한국축산식품학회
• 저자명: Sang Buem Cho Woo Kyung Chang Yun Jung Kim Hyung In Moon Jong Won Joo In Soo Choi Kun Ho Seo Soo Ki
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제30권 제5호, 764~772쪽, 전체 9쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2010.10.31

영문 초록

Staphylococcus aureus lipase is regarded as a virulence factor. The response of lipase activity to various factors can provide important insights concerning the prevention of S. aureus during meat fermentation. This study was conducted to evaluate the main effects of nutrients used in culture media, and their combined effects on the inhibition of lipase activity and cell growth of pathogenic S. aureus SK1593 isolated from fermented pork meat. A Plackett-Burman design was used to evaluate the main effects of variables, including olive oil, soybean oil, grapeseed oil, sesame oil, CuSO4, MgCl2, KNO3, CaCl2, and KCl. Significant negative effects on lipase activity were detected with soybean oil, grapeseed oil, KNO3, and CaCl2. Additionally, these nutrients were further selected as variables for the investigation of their combined effect on lipase activity, via response surface methodology. In order to confirm the regression model, a situation that only inhibits lipase activity was simulated. The predicted lipase activity and cell growth of the simulated situation were 14.0 U/mL and 9.6 log10 (CFU/mL), respectively, and the estimated value of those in the same medium showed 15.14 U/mL and 9.4 log10(CFU/mL) respectively. The lipase activity of the simulated medium was inhibited approximately 5-fold as compared to the basal medium, but no significant differences in cell counts were noted to exist between the basal and simulated media. These results suggest that soybean oil, grapeseed oil, KNO3, and CaCl2 can be used to inhibit the growth of pathogenic S. aureus during the process of meat fermentation.

키워드

Staphylococcus aureus SK1953 Lipase activity Plackett-Burman design response surface model