PKC-Independent Stimulation of Cardiac Na+/Ca2+ Exchanger by Staurosporine

• 영문명: PKC-Independent Stimulation of Cardiac Na⁺/Ca²⁺ Exchanger by Staurosporine
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Tong Mook Kang
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제5호, 259~266쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

[Ca²⁺]_i transients by reverse mode of cardiac Na⁺/Ca²⁺ exchanger (NCX1) were recorded in fura-2 loaded BHK cells with stable expression of NCX1. Repeated stimulation of reverse NCX1 produced a long-lasting decrease of Ca²⁺ transients ( rundown ). Rundown of NCX1 was independent of membrane PIP₂ depletion. Although the activation of protein kinase C (PKC) was observed during the Ca²⁺ transients, neither a selective PKC inhibitor (calphostin C) nor a PKC activator (PMA) changed the degrees of rundown. By comparison, a non-specific PKC inhibitor, staurosporine (STS), reversed rundown in a dose-dependent and reversible manner. The action of STS was unaffected by pretreatment of the cells with calphostin C, PMA, or forskolin. Taken together, the results suggest that the stimulation of reverse NCX1 by STS is independent of PKC and/or PKA inhibition.

키워드

Na⁺/Ca²⁺ exchange Rundown Staurosporine Protein kinase C BHK cell