Netrin-1 Specifically Enhances Cell Spreading on Fibronectin in Human Glioblastoma Cells

• 영문명: Netrin-1 Specifically Enhances Cell Spreading on Fibronectin in Human Glioblastoma Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Hyun Kyoung Lee In Ae Seo Yoon Kyung Shin Sang Hwa Lee Su-Young Seo Duk Joon Suh Hwan Tae Park
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제5호, 225~230쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

Netrins are secreted molecules and involved in axon guidance, cell migration and tumor development. Recent studies revealed that netrins perform novel functions in such processes as epithelial development and angiogenesis without operating through the classical netrin receptors, DCC (Deleted in Colorectal Cancer) and Unc5h. In the present study, we investigated the roles of netrin-1 and its receptors in cell spreading of human glioblastoma cells, and found that netrin-1 haptotactically enhanced fibronectin-induced cell spreading and focal adhesion formation in U373 glioblastoma cells. Netrin-1 binding to the U373 cell membrane was blocked by an antibody against Ձv integrin subunit, but not by an anti-DCC or anti-Unc5h antibody. In addition, enhancement of the fibronectin response by netrin-1 was abrogated by a function blocking antibody against integrin ՁvՂ3. Since the Ձv subunit of the integrin family plays an important role in the pathophysiological aspects of cell migration, including tumor angiogenesis and metastasis, our data provide important insight into the molecular mechanism of netrin function.

키워드

Netrin Fibronectin Spreading Integrin alpha v Glioblastoma