Single-Channel Recording of TASK-3-like K+ Channel and Up- Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons

• 영문명: Single-Channel Recording of TASK-3-like K⁺ Channel and Up- Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Inseok Jang Jun-Ho La Gyu-Tae Kim Jeong-Soon Lee Eun-Jin Kim Eun-Shin Lee Su-Jeong Kim Jeong-Min Seo
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제5호, 245~252쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K⁺ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K⁺ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of TASK-3-like K⁺ channel was 33.0±0.1 pS at −60 mV, and TASK-3 activity fell by 65±5% when the extracellular pH was changed from 7.3 to 6.3, indicating that the DRG K⁺ channel is similar to cloned TASK-3 channel. TASK-3 mRNA and protein levels in brain, spinal cord, and DRG were significantly higher in injured animals than in sham-operated ones. These results indicate that TASK-3 channels are expressed and functional in DRG neurons and the expression level is up-regulated following SCI, and suggest that TASK-3 channel could act as a potential background K^＋ channel under SCI-induced acidic condition.

키워드

Two-pore domain K⁺ channel Dorsal root ganglion Spinal cord injuries Acidosis