토끼 적혈구막의 (Na++K+-ATPase의 active center에 관한 연구

• 영문명: Studies on Active Center of (Na⁺+K⁺-ATPase in Rabbit Red Cell Membranes
• 발행기관: 대한생리학회
• 저자명: 임보상(Lim, Bo-Sang)
• 간행물 정보: 『대한생리학회지』제9권 제1호, 1~12쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1975.06.30

영문 초록

The present experiments were carried out to investigate the active center of sodium and potassium ion activated adenosine triphosphatase. An ATPase, activated by sodium ion Plus potassium ion in the presence of magnesium ion, and inhibited by ouabain, has been obtained from rabbit red cell ghosts. The ATPase activity was measured by inorganie phosphate released from ATP. From this values of the measured inorganic phosphate, the activity of ATPase was calculated. The following results were observed. 1. The activity of (Na⁺+K⁺)-ATPase is inhibited by ouabain. This effect may not be due to an effect on sulfhydryl groups, amino groups, carboxyl groups, imidazole groups and hydroxyl groups. 2. The (Na⁺+K⁺)-activated enzyme system is inhibited by p-chloromercuribenzoate and by d nitroflurobenzene, and this effect may be due to an effect on sulfhydryl groups. These results indicate that the sulfhydryl groups is attached to sodium-potassium dependent adenosine triphosphate, an aspect of the pump. 3. The (Na⁺+K⁺)-activated enzyme system is inhibited by maleic anhydride and this inhibition is reversed by lysine. This Seems to indicate that the active center of this enzyme is the amino groups. 4. The (Na⁺+K⁺)-activated enzyme system is inhibited by iodoacetamide and this inhibition is reversed by the simultaneous present of cysteine and aspartic acid in the suspension medium. This result indicates that this enzyme contains sulfhydryl groups and carboxyl groups. 5. The (Na⁺+K⁺)-ATPase activity is accelerated by adrenaline and this effect is abolished by aspartic acid. This effect of aspartic acid indicate that carboxyl group might be involved in the hydrolysis of ATP by the enzyme system. On the hydrolysis of ATP by the enzyme system. On the basis of these experiments it f¡¤as suggested that the active center of (Na⁺+K⁺)-activated ATPase contains sulfhydryl groups, amino groups and carboxyl groups.