Optimization of Shoot Induction, Histological Study and Genetic Stability of in vitro Cultured Pisum sativum cv. ‘Sparkle’

• 발행기관: 한국자원식물학회
• 저자명: Vipada Kantayos Chang-Hyu Bae
• 간행물 정보: 『한국자원식물학회지』제32권 제1호, 19~28쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.02.28

영문 초록

An efficient shoot regeneration condition for pea cv. ‘Sparkle’ was developed by using optimum explant, plantgrowth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed thehighest on two kinds of shoot induction media (MSB5 media containing 2 ㎎/L BA and a combination of 2 ㎎/L BA and 1㎎/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 ㎎/L BA solutionwas found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division andproto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlierthan 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitroregenerated plants showed polymorphism with 8.3% compared with their mother plants.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgement

References

키워드

Cotyledonary node Genetic Stability Histological study Pea Shoot Regeneration