Simultaneous detection of aflatoxin B1, deoxynivalenol, and fumonisin B1 in blood with liquid chromatography-tandem mass spectrometry

• 발행기관: 한국예방수의학회
• 저자명: Kwang-Nam Kim Soohee Kim Hyobi Kim Seo-Il Yang Heesu Kim Jae-Young Song Sung-Won Park
• 간행물 정보: 『Journal of Preventive Veterinary Medicine』Vol.39, No.3, 108~113쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2015.09.30

영문 초록

Mycotoxins, such as aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FMB1), ochratoxin A, T2 toxin, and zearalenone, are found in numerous vegetables. Mycotoxin accumulation in food and feed poses serious health risks to humans and animals because of carcinogenic, mutagenic, teratogenic, and toxic properties. In addition, mycotoxins cause large economic losses in commercial crop production, food and feed processing, and animal husbandry worldwide. In this study, an analytical method for the simultaneous analysis of the levels of AFB1, DON, and FMB1 in cow blood with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. AOZTM and Myco6in1TM multitoxin immunoaffinity columns and an OasisTM reversed-phase solid-phase extraction Hydrophilic-Lipophilic-Balanced columns were used to purify and concentrate the blood samples. Extracts that contained AFB1, DON, and FMB1 had average recovery of 64.0%, 98.0%, and 89.9%, respectively. In conclusion, we used LC-MS/MS to detect several important toxicological mycotoxins in cow blood. The multimycotoxin method, which detected and quantified the levels of AFB1, DON, and FMB1 can be used in animal pilot studies to monitor simultaneous exposure to major mycotoxins.

목차

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

REFERENCES

키워드

Mycotoxins cow blood multitoxin immunoaffinity column LC-MS/MS