광귤나무(Citrus aurantium) 열매에서 분리된 쿠마린의 iNOS 억제효과

• 영문명: Inhibitory effects of coumarins from the fruit of Citrus aurantium against iNOS activity
• 발행기관: 대한약학회
• 저자명: 김범선(Beom sun Kim) 정의길(Eui Gil Jung) 윤형선(Hyung Sun Youn) 황경아(Kyung A Hwang) 한만덕(Man Deuk Han) 유연호(Yon ho Yu) 남궁우(Kung Woo Nam)
• 간행물 정보: 『약학회지』제61권 제5호(2017년), 267~273쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2017.10.30

영문 초록

Citrus fruits have been used as an edible fruit, and also as a traditional medicine, since ancient times. A crude methanol extract of Citrus aurantium was shown to suppress inducible proinflammatory cytokines, but its constituents were not known. In this study, we isolated four furanocoumarins, bergapten (1), imperatorin (2), isoimperatorin(3), and phellopterin (4), and one 7-geranyloxycoumarin, auraptene (5), from a methanol extract of dried Citrus aurantium fruit. The structures of these compounds were elucidated by extensive application of spectroscopic techniques, including 2D NMR spectroscopy and mass spectrometry. All of the isolated compounds were evaluated for their ability to inhibit the production of proinflammatory cytokine inducible nitric oxide synthase (iNOS) in lipopolysaccharide-activated RAW264.7 cells. Compounds 2−5 significantly suppressed iNOS production but compound 1 had no iNOS inhibitory effects. These results indicate that at least three furanocoumarins and one 7-geranyloxycoumarin are involved in the suppressive effects of citrus fruits against the production of inducible inflammatory cytokines.

목차

Abstract

실험 방법(Experimental Methods)

결과 및 고찰(Results and Discussion)

결론(Conclusion)

감사의 말씀(Acknowledgement)

References

키워드

Citrus aurantium furanocoumarins 7-geranyloxycoumarin iNOS inflammation