보이차(Pu-erh tea)의 HaCaT 세포보호 및 HCT116 세포사멸 효과

• 영문명: Effect of Pu-erh tea on UVB-induced oxidative damage in human HaCaT and cytotoxicity in HCT116 human colon cancer cells
• 발행기관: 한국차학회
• 저자명: 권태은(Tae Eun Guon) 정지승(Ji Seung Jeong) 정하숙(Ha Sook Chung)
• 간행물 정보: 『한국차학회지』제22권 제4호, 115~121쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.12.30

영문 초록

The present study was performed to investigate the biological activity of fermented Pu-erh (FP) and raw Pu-erh (RP) tea extractives. In this study, we examined the antioxidants with the DPPH and ABTS assays and conducted an apoptotic analysis with the MTT assay and western blotting experiments. The total phenolic contents in FP and RP were 38.59 ± 15.23 and 36.83 ± 6.60 mg GAE/g dry weight, respectively. The effects of FP and RP on the apoptotic pathway were also investigated. It was found that the levels of Bcl-2 decreased, whereas those of Bax and cleaved caspase-9, caspase-3 and PARP increased in a dose-dependent manner. In addition, we investigated the protective effects of FP and RP against ultraviolet B-induced oxidative damage in human keratinocytes (HaCaT cells). To induce oxidative stress in the HaCaT cells, they were irradiated with UVB at 30 mJ/㎠. FP and RP was found to be reduced phosphorylation of IkBα and IKKα/β, block nuclear factor-kB p65 nuclear translocation, and strongly suppress the pro-inflammatory mediators.

목차

Abstract

서론

실험재료 및 방법

결과 및 고찰

적요

참고문헌

키워드

Pu-erh tea UVB antioxidant anti-proliferative anti-inflammatory HaCaT HCT116