충남지역 지하수에서 분리한 총대장균군 양성시료 중수인성 병원균의 분석

• 영문명: Analysis of Waterborne Pathogenic Bacteria among Total Coliform Positive Samples in the Groundwater of Chungcheongnam-do Province, Korea
• 발행기관: 한국환경보건학회
• 저자명: 유정호(Jungho Yu) 왕창근(Changkeun Wang) 신인철(Inchul Shin) 김동욱(Donguk Kim) 박귀성(Kwisung Park)
• 간행물 정보: 『한국환경보건학회지』제42권 제3호, 189~195쪽, 전체 7쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2016.06.30

영문 초록

Objectives: To ensure the microbiological safety of groundwater, it was confirmed whether waterborne pathogenic bacteria in groundwater samples tested positive for total coliforms in the Chungcheongnam-do Province region. Methods: Total colony counts, total coliforms and fecal coliforms were tested according to the process mandated by the drinking water quality testing standards of Korea. DNA was extracted from the samples, tested positive for total coliforms, and then subjected to real-time PCR to detect waterborne pathogenic bacteria. Results: A total of 115 samples were inadequate for drinking water. Thirty-one cases (27%) showed positive for fecal coliforms and nine cases (7.8%) showed total colony counts exceeding drinking water standards. Twentyseven cases (23.5%) showed three items (total colony counts, total coliforms and fecal coliforms). Using the realtime PCR method, waterborne pathogens were detected in 57 cases (49.6%) in 115 samples. Seventy-eight cases of waterborne pathogenic bacteria were detected (including duplications): 27 cases of pathogenic E. coli (EPEC (19), ETEC (5), EHEC (1), EAEC (1) and EIEC (1)); 45 of Bacillus cereus; two of Yersinia spp.; two of Salmonella spp.; one of Staphylococcus aureus; one of Clostridium perfringens. Conclusion: The real-time PCR method can offer rapid and accurate detection of waterborne pathogenic bacteria. Therefore, this assay could be an alternative to conventional culture methods and can further ensure the microbiological safety of groundwater.

목차

I. 서 론

II. 재료 및 방법

III. 결 과

IV. 고 찰

V. 결 론

키워드

Groundwater real-time PCR total coliforms waterborne pathogenic bacteria