Xenografted Tumorigenesis in the oral vestibule of nude mice by Snail transfection:

• 발행기관: 대한구강악안면외과학회
• 저자명: Moon-Key Kim Eun-Ha Lee Jin Kim Jong-In Yook In-Ho Cha
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면회과학회지 제35권 제4호, 199~204쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2009.08.30

영문 초록

Purpose: The purpose of this study is to investigate the epithelial-mesenchymal transition (EMT) induced by Snail transcription factor and Snailtransfected in vivo tumors with histopathological features. Materials and methods: We induced in vivo xenografted tumorigenesis in the oral vestibules of nude mice by a Snail transfected HaCaT cell line and investigated morphological and immunohistochemical features in Snail expressive tumors. Results: We identified tumor masses in 14 out of 15 nude mice in the HaCaT-Snail cell inoculation group, but no tumors were present in any of the HaCaT cell inoculation group. Induced tumors showed features of poorly differentiated carcinoma with invasion to neighboring muscles and bones. The HaCaT-Snail tumors showed decreased expressions of E-cadherin and cytokeratin, but showed increased expressions of vimentin and N-cadherin. Discussion: The Snail transfected xenograft can improve productivity of malignant tumors, show various histopathological features including invasive growth, and aid in the investigation of tumor progression and the interaction with surrounding tissues

키워드

E-cadherin neoplastic cell transformation Snail transcription factor xenograft model