This study aimed to identify the effects of contract-relax proprioceptive neuromuscular facilitation (PNF) stretching and ankle joint static stretching on the range of motion of the ankle joint, dynamic and static balance, and gait abilities of chronic stroke patients. The study participants consisted of 22 stroke patients, and the range of the motion of the ankle joint, calf muscle stiffness, static balance, dynamic balance, and walking abilities were assessed during a pre-test. The first intervention was a 10-min session of contract-relax PNF stretching, and a post-test identical to the pre-test was performed immediately after the intervention. Three days later, all participants underwent the same pre-test, participated in the second intervention of a 10-min session of static stretching, and then underwent the same post-test. After participating in the contract-relax PNF stretching and static stretching, calf muscle stiffness significantly decreased, and the range of motion of the ankle joint also improved. In addition, both types of stretching were significantly effective on dynamic balance abilities, significantly decreasing the static balance abilities. However, no significant differences were observed from both types of stretching on the gait abilities. Therefore, ankle stretching and combined therapeutic exercises are recommended for stroke patients in order to improve the static balance abilities when standing up.