Chemometrics Assisted-Liquid Chromatography (LC)-Orbitrap High Resolution Mass Spectrometry (HRMS) Non-Targeted Metabolomics for Identification of Dog Meat in Beef Sausages

• 발행기관: 한국축산식품학회
• 저자명: Anjar Windarsih Nor Kartini Abu Bakar Abdul Rohman Ida Musfiroh Dachriyanus
• 간행물 정보: 『Food Science of Animal Resources』제45권 제4호, 1176~1190쪽, 전체 15쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2025.07.15

국문 초록

The presence of non-halal meats in declared halal meat products is truly prohibited to be consumed, especially for Muslim consumers. The objective of this study was to identify dog meat (DM) adulteration in beef sausage (BS) using an untargeted metabolomics technique based on liquid chromatography-high resolution mass spectrometry (LC-HRMS) Orbitrap incorporated with chemometrics tools. Chemometrics of principal component analysis (PCA), partial least square-discriminant analysis (PLS-DA), PLS, and orthogonal PLS was used to manage the metabolomics data. PCA could differentiate the authentic BS and that adulterated with DM. In addition, PLS-DA successfully classified BS and BS adulterated with DM according to their classes with r2X=0.815, r2Y=0.950, and Q2=0.582, repectively. Metabolites of trans-10-heptadecenoic acid, N-stearoyltyrosine, L-gammaglutamyl- L-leucine, 1-(beta-D-ribofuranosyl) thymine, adenosine, (3beta,24R,24’R)-fucosterol epoxide, acetyl-L-carnitine, isoleucine, diisobutyl adipate, L-tyrosine, carnosine, and DLglutamine were found as discriminating metabolites with variable importance for projection value of >2.0 for discriminating BS from DM adulteration. In addition, orthogonal PLS using variables of discriminating metabolites was successfully used to predict DM levels in BS with r2 of 0.9995, root mean square error of estimation of 0.88%, and root mean square error of prediction of 1.63%. It can be concluded that the untargeted metabolomics approach using LC-HRMS assisted with chemometrics is potential tools to be used for the authentication of BS from DM adulteration. In the future, research on larger samples and method standardization is highly urgent to ensuring the reproducibility of this method.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

References

키워드

beef sausage chemometrics dog meat halal authentication liquid chromatographyhigh resolution mass spectrometry (LC-HRMS) metabolomics