Exploring the Modulation of Extracellular Metabolites in Different Listeria monocytogenes Strains under Cold-Stress

• 발행기관: 한국축산식품학회
• 저자명: Hyun-Jun Kim Hye-Jin Kim Cheorun Jo
• 간행물 정보: 『Food Science of Animal Resources』제45권 제4호, 1114~1128쪽, 전체 15쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2025.07.15

국문 초록

This study investigated the modulation of extracellular metabolites in Listeria monocytogenes NCCP 15743 (L5), NCCP 16594 (L6), and ATCC 19111 (L9) strains in cold-stressed culture. The strains were cultured in Mueller Hinton broth at 8℃ for 22 d. Extracellular metabolites were extracted at five growth phases (initial, lag, log, early saturate, and saturate) of each strain. Under cold-stress, growth phases of L5 and L6 exhibited similarities, while L9 displayed a distinct pattern. The change in extracellular metabolites under cold-stress was dependent on growth phase and strain. The presence of L. monocytogenes was distinguished based on the concentrations of trehalose, isoleucine, arginine, and phenylalanine. During extended cold-stressed culture, all strains enhanced two metabolic pathways at the lag and log phases: energy metabolism (trehalose, lactate, propanoate, acetate, ethanol, and formic acid) and glutathione-related metabolism (acetate, histidine, arginine, proline, glutamate, glycine, serine, and methionine). The expression of these extracellular metabolites provides crucial insights into the complex metabolic adaptations of L. monocytogenes during cold-stress culture. This study introduces a distinctive approach to identifying L. monocytogenes under cold-stress, offering potential application for safety enhancement in the food industry.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

References

키워드

extracellular metabolites multivariate analysis culture media foodborne pathogens detection method nuclear magnetic resonance spectroscopy