돌외 잎의 기능성 진세노사이드 성분에 대한 추출 조건 최적화

• 영문명: Optimization of Extraction Conditions for Functional Ginsenosides in Gynostemma pentaphyllum Leaves
• 발행기관: 한방비만학회
• 저자명: 김도형 고준휘 이효진 정지훈 양병욱
• 간행물 정보: 『대한한방비만학회지』제25권 제1호, 1~8쪽, 전체 8쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2025.06.30

국문 초록

Objectives: The aims of this study was to evaluate the ginsenoside content in the leaves of Gynostemma pentaphyllum (Cucurbitaceae), under different extraction conditions. As a dammarane triterpene-type derivative, G. pentaphyllum contains ginsenosides similar to those in ginseng, making it a potential source for herbs as functional food ingredients with anti-obesity effects. Methods: Ginsenosides were isolated from G. pentaphyllum extracts using both water and ethanol at different concentrations and duration. Water extractions were performed at 100 ℃ for 10 and 24 hours. Solvent extractions used 50%, 70%, and 95% ethyl alcohol. Ginsenoside contents were analyzed in each extract by high performance liquid chromatography. Results: Water extraction of G. pentaphyllum leaves at 100 ℃ for 24 hours (GPW-24) showed the highest total content of ginsenoside. However, 10-hour extraction (GPW-10) was yielded the highest concentrations of ginsenoside Rh1 (0.886%) and Rc (0.566%). In solvent extraction, total ginsenosides were highest in 95% ethanol, and were followed by 50%, 70%, and water. Ginsenoside Rc was abundant in both 95% (4.745%) and 50% ethanol (4.769%) extracts. Conclusions: The optimal water extraction condition for obtaining functional ingredients is 10-hour extraction at 100 ℃. The best solvent extraction condition is using 95% ethanol, suggesting effective strategies for utilizing G. pentaphyllum in functional herbal ingredients with anti-obesity effects.

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키워드

Gynostemma pentaphyllum Herbal medicine Ginsenoside Rh1 Ginsenoside Rc