쉽싸리의 기내 마디배양 시 탄소급원의 종류와 농도가 식물체 재분화 및 증식에 미 치는 영향

• 영문명: Effect of carbon source and concentration on in vitro regeneration and propagation in Lycopus lucidus by node culture
• 발행기관: (사)한국식물생명공학회
• 저자명: 이나념(Na-nyum Lee) 김지아(Ji Ah Kim) 김용욱(Yong Wook Kim) 김태동(Tae Dong Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』45권 2호, 131~139쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.06.30

영문 초록

We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.

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재료 및 방법

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References

키워드

Lycopus lucidus Turcz. ex Benth. Carbon source Regeneration Acclimatization