탄소급원에 의한 얌의 기내 비대근 형성과 순화

• 영문명: Microtuberization and Acclimatization in the Dioscorea cayenensis Thunb. by the Carbon Source
• 발행기관: (사)한국식물생명공학회
• 저자명: 이나념(Na Nyum Lee) 김지아(Ji Ah Kim) 김용욱(Yong Wook Kim) 김태동(Tae Dong Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』45권 2호, 125~130쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.06.30

영문 초록

In this experiment, we investigated the effects of various carbon sources and concentrations on the microtuber induction and acclimatization of the yam (Dioscorea cayenensis). First, the effects of the in vitro carbon sources and concentrations on the microtuber induction were examined. The highest efficiency of the microtuber induction was obtained in the 7% sucrose treatment, whereas the glucose treatment shows no effect on the microtuber formation. Secondly, the effects of the survival rate and the microtuber formation rate after the acclimatization were examined. The diameter (6.1 mm) and fresh weight (0.5g) of the tuberous root are the highest in the pretreatment of the 7% sucrose. Although the survival rate of the pretreatment of the low concentration sucrose (3% sucrose) is 100 %, the growth and development were inhibited. These results suggest the 7% sucrose treatment is appropriate for the yam microtuber formation and acclimatization. In addition, this protocol could be used for the propagation of virus- or disease-free clones and the multiplication of elite yam cultivars.

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키워드

Dioscorea cayenensis microtuberization carbohydrate