엉겅퀴의 ITS 영역 염기서열 분석을 통한 특이적 SNP 분자마커의 개발

• 영문명: Development of specific SNP molecular marker from Thistle using DNA sequences of ITS region
• 발행기관: (사)한국식물생명공학회
• 저자명: 이신우(Shin-woo Lee) 이수진(Soo Jin Lee) 김윤희(Yun-hee Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』45권 2호, 102~109쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.06.30

영문 초록

Thistle is a perennial plant that is widely used for medicinal purposes. Information on the genetic diversity of thistle populations are great important for their conservation and germ plasmic utilization. Although thistle is an important medicinal plant species registered in South Korea, no molecular markers are currently available to distinguish them from other similar species from different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from the nuclear ribosomal DNA internal transcribed spacer (ITS) regions of genomic sequences to identify distinct Korean-specific thistle species via an amplification refractory mutation system (ARMS)-PCR and high resolution melting (HRM) curve analyses. We performed molecular authentication of four different kinds of thistle species from different regions using DNA sequences in the ITS intergenic region. We also developed a quantitative PCR assay using species-specific ITS primers, which allowed us to estimate the ratio of Korean-specific thistle species using varying ratios of mixed genomic DNA templates from the two species. The SNP markers developed in this study are useful for rapidly identifying specific thistle species from different countries.

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키워드

ARMS-PCR HRM curve analysis Nuclear ribosomal DNA internal transcribed spacer regions Single nucleotide polymorphisms Thistle