편육과 브로콜리싹에서 Salmonella spp. 검출을 위한 배지법과 Real-time PCR 및 신속 검사키트(VIDAS(R))의 비교검증

• 발행기관: 한국축산식품학회
• 저자명: 현지연 황인균 곽효선 박종석 허석 최인수 박찬규 서건호
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제29권 제4호, 506~512쪽, 전체 7쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2009.10.31

영문 초록

Salmonellosis is an important worldwide foodborne infectious disease that is transmitted by many food vehicles including raw and processed animal products and fresh produce. In this study, the effectiveness of automated ELISA (VIDAS(R)) and real-time PCR in the detection of Salmonella spp. in steamed pork and raw broccoli sprouts was evaluated by comparing their results with those of a conventional culture method. Bulk samples (500 g) of steamed pork and raw broccoli sprouts were inoculated with various levels of Salmonella and divided into 20 samples (25 g each). All the samples, including the controls, were analyzed using a conventional culture method, VIDAS(R), and real-time PCR to detect the presence of Salmonella. In addition, the levels of background flora in the steamed pork and the raw broccoli sprouts were determined. In the steamed pork that contained less than 100 CFU/g of aerobic bacteria, all three methods detected low levels of Salmonella without a statistical difference in their performance. In the broccoli sprouts with high quantities of background flora (ca. 6.7×10(7) CFU/g), however, all three methods were unable to detect low levels of Salmonella, and real-time PCR and VIDAS(R) more sensitively detected Salmonella than the culture method, with significant statistical differences. In conclusion, VIDAS(R) and real-time PCR could be superior to conventional culture methods in detecting Salmonella in food with high levels of background flora.

키워드

Salmonella culture method VIDAS(R) Real-time PCR