Spontaneous Electrical Activity of Cultured Interstitial Cells of Cajal from Mouse Urinary Bladder

• 영문명: Spontaneous Electrical Activity of Cultured Interstitial Cells of Cajal from Mouse Urinary Bladder
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Sun-Ouck Kim Han-Seong Jeong Sujeong Jang Mei-Jin Wu Jong Kyu Park Han-Yi Jiao Jae Yeoul Jun Jong-Se
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제17권 제6호, 531~536쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2013.01.01

영문 초록

Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular Ca²⁺ ([Ca²⁺]_i) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased [Ca2⁺]_i oscillations and basal Ca²⁺ levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.

키워드

Interstitial cells of Cajal Spontaneous electrical activities Urinary bladder