Potassium Currents in Isolated Deiters Cells of Guinea Pig

• 영문명: Potassium Currents in Isolated Deiters Cells of Guinea Pig
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Jong Woo Chung Eui Chol Nam Won Tae Kim Jae Boum Youm Chae Hun Leem
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제17권 제6호, 537~546쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2013.01.01

영문 초록

Deiters cells are the supporting cells in organ of Corti and are suggested to play an important role in biochemical and mechanical modulation of outer hair cells. We successfully isolated functionally different K⁺ currents from Deiters’ cells of guinea pig using whole cell patch clamp technique. With high K⁺ pipette solution, depolarizing step pulses activated strongly outward rectifying currents which were dose-dependently blocked by clofilium, a class III anti-arrhythmic K⁺ channel blocker. The remaining outward current was transient in time course whereas the clofilium-sensitive outward current showed slow inactivation and delayed rectification. Addition of 5 mM tetraethylammonium (TEA) further blocked the remaining current leaving a very fast inactivating transient outward current. Therefore, at least three different types of K⁺ current were identified in Deiters cells, such as fast activating and fast inactivating current, fast activating slow inactivating current, and very fast inactivating transient outward current. Physiological role of them needs to be established.

키워드

Dieters’ cell Hearing Inner ear Organ of Corti Pharmacology Potassium channels