Lysophosphatidylcholine Increases Ca2+ Current via Activation of Protein Kinase C in Rabbit Portal Vein Smooth Muscle Cells

• 영문명: Lysophosphatidylcholine Increases Ca²⁺ Current via Activation of Protein Kinase C in Rabbit Portal Vein Smooth Muscle Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Seungsoo Jung Youngho Lee Sungsik Han Youngwhan Kim Taiksang Nam Ducksun Ahn
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제1호, 31~34쪽, 전체 4쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

Lysophosphatidylcholine (LPC), a metabolite of membrane phospholipids by phospholipase A₂, has been considered responsible for the development of abnormal vascular reactivity during atherosclerosis. Ca²⁺ influx was shown to be augmented in atherosclerotic artery which might be responsible for abnormal vascular reactivity. However, the mechanism underlying Ca²⁺ influx change in atherosclerotic artery remains undetermined. The purpose of the present study was to examine the effects of LPC on L-type Ca²⁺ current (I_Ca(L)) activity and to elucidate the mechanism of LPC-induced change of I_Ca(L) in rabbit portal vein smooth muscle cells using whole cell patch clamp. Extracellular application of LPC increased I_Ca(L) through whole test potentials, and this effect was readily reversed by washout. Steady state voltage dependency of activation or inactivation properties of I_Ca(L) was not significantly changed by LPC. Staurosporine (100 nM) or chelerythrine (3ՌM), which is a potent inhibitor of PKC, significantly decreased basal I_Ca(L), and LPC-induced increase of I_Ca(L) was significantly suppressed in the presence of PKC inhibitors. On the other hand, application of PMA, an activator of PKC, increased basal I_Ca(L) significantly, and LPC-induced enhancement of I_Ca(L) was abolished by pretreatment of the cells with PMA. These findings suggest that LPC increased I_Ca(L) in vascular smooth muscle cells by a pathway that involves PKC, and that LPC-induced increase of I_Ca(L) might be, at least in part, responsible for increased Ca²⁺ influx in atherosclerotic artery.

키워드

Lysophosphatidylcholine Ca²⁺ current Protein kinase C Vascular smooth muscle