Effects of TGF-Ղ1 Ribbon Antisense on CCl4-induced Liver Fibrosis

• 영문명: Effects of TGF-Ղ1 Ribbon Antisense on CCl₄-induced Liver Fibrosis
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Kyung-Oh Doh
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제12권 제1호, 1~6쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2008.01.01

영문 초록

Ribbon-type antisense oligonucleotide to TGF-<FONT FACE= HJ중명조 >Ղ1 (TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS) was designed and tested to prevent or resolve the fibrotic changes induced by CCl₄ injection. When Hepa1c1c7 cells were transfected with TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS, the level of TGF-<FONT FACE= HJ중명조 >Ղ1 mRNA was effectively reduced. TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS, mismatched RiAS, and normal saline were each injected to mice via tail veins. When examined for the biochemical effects on the liver, TGF-<FONT FACE= HJ중명조 >Ղ1 mRNA levels were significantly reduced only in the TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS-treated group. The results of immunohistochemical studies showed that TGF-<FONT FACE= HJ중명조 >Ղ1 RiAS prevented the accumulation of collagen and <FONT FACE= HJ중명조 >Ձ-smooth muscle actin, but could not resolve established fibrosis. These results indicate that ribbon antisense to TGF-<FONT FACE= HJ중명조 >Ղ1 with efficient uptake can effectively prevent fibrosis of the liver.

키워드

Transforming growth factor-Ղ 1 Liver cirrhosis Ribbon antisense Cationic peptide