The Hyperthermic Effect of Nitric Oxide in Central Nervous System

• 영문명: The Hyperthermic Effect of Nitric Oxide in Central Nervous System
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Jae-Kyung Jung Uy Dong Sohn Seok-Yong Lee
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제5권 제1호, 93~98쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2001.01.01

영문 초록

The precise mechanism of set-point regulation in hypothalamus was not elucidated. Nitric oxide synthases (NOS) were detected in hypothalamus, however, the roles of NO in hypothalamus was not fully studied. So, we tested the effects of NO on body temperature because preoptic-anterior hypothalamus was known as the presumptive primary fever-producing site. NO donor sodium nitroprusside (SNP, 4 nmol, i.c.v.) elicited marked febrile response, and this febrile response was completely blocked by indomethacin (a cyclooxygenase inhibitor). But, ODQ (selective guanylate cyclase inhibitor, 50μg, i.c.v.) did not inhibit fever induced by SNP. The cyclic GMP analogue dibutyryl-cGMP (100μg, i.c.v.) induced significant pyreses, which is blocked by indomethacin. N^G-nitro-L-arginine methyl ester (L-NAME, non selective NOS inhibitor) inhibited fever induced by interleukin-1β (IL-1β, 10 ng, i.c.v.), one of endogenous pyrogens. These results indicate that NO may have an important role, not related to stimulation of soluble guanylate cyclase, in the signal pathway of thermoregulation in hypothalamus.

키워드

Nitric oxide Nitroprusside Pyrexia i.c.v. injection ANOVA analysis of variance DTT dithiothreitol Grp glucose-regulated protein HSP heat shock family of stress proteins Hsp heat shock protein PDBu phorbol 12,13-dibutyrate PKC protei