Pulse Exposure to Ethanol Augments Vascular Contractility Through Stress Response

• 영문명: Pulse Exposure to Ethanol Augments Vascular Contractility Through Stress Response
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Eun-Kyoung Yang InKyeom Kim
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제5권 제1호, 47~53쪽, 전체 7쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2001.01.01

영문 초록

Drinking excessive alcohol has been recognized as a risk factor for hypertension. However, the mechanism by which alcohol intake causes hypertension still remains elusive. We tested the hypothesis that ethanol itself acts as a stress factor on vasculature and indirectly modulates vascular contractility. After end of exposure to 1, 2.5 and 5% ethanol for 45 min, rat aortic strips were subjected to contractile responses, immunoblot for Hsp70 and the measurement of levels of myosin light chain phosphorylation. Exposure to 5% ethanol not only augmented contractions to KCl or phenylephrine, but also increased expression of Hsp70 and the levels of myosin light chain phosphorylation. There were no significant differences in contractions produced by 1μmol/L phorbol 12,13-dibutyrate, a protein kinase C activator, whether the tissues were exposed to 5% ethanol or not. This is the first report to show that even short exposure to ethanol has a delayed effect to increase vascular smooth muscle contractility through a modulation of thick filament regulation. It may be a mechanism by which ingestion of alcohol induces hypertension.

키워드

Ethanol Hsp 70 family Hypertension Myosin Light Chains Phosphorylation Contraction ANOVA analysis of variance DTT dithiothreitol Grp glucose-regulated protein HSP heat shock family of stress proteins Hsp heat shock protein PDBu phor