Interaction between Cholecystokinin and Secretin in Isolated Rat Pancreatic Acini

• 영문명: Interaction between Cholecystokinin and Secretin in Isolated Rat Pancreatic Acini
• 발행기관: 대한생리학회
• 저자명: Yoon, Shin-Hee Hahn, Sang-June Sim, Sang-Soo Rhie, Duck-Joo Song, In-Young Baek, Hye-Jung Kim, Myung
• 간행물 정보: 『대한생리학회지』제29권 제2호, 243~250쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1995.12.31

영문 초록

A possible potentiation between cholecystokinin (CCK) and secretin in amylase secretion from isolated rat pancreatic acini was investigated. Combined treatment of acini with secretin and CCK at low concentrations, which are known to be physiological, resulted in enzyme secretion larger than the arithmetic sum of their separate effects. Such a potentiating effect also occurred between secretin and A23187 (Ca ionophore), between forskolin (adenylate cyclase activator) and CCK, and between forskolin and A23187. Staurosporin (protein kinase C inhibitor) and W7 (calmodulin antagonist) inhibited markedly the potentiated amylase release induced by the agonists, but KT5720 (protein kinase A inhibitor) did not affect the potentiated amylase release. Therefore, we concluded that the action of CCK in a physiological concentration is potentiated by secretin in a physiological concentration range and vice versa, and that the intracellular mechanism necessary for the potentiation is associated with Ca²⁺. However, it is uncertain what mechanisms are involved in potentiation of amylase release after CAMP and Ca²⁺.

키워드

Calcium cAMP Cholecystokinin Pancreatic amylase Potentiation Secretin